Journal: Journal of Clinical Medicine

Article Title: Urinary Proteomics Reveals Promising Biomarkers in Menstrually Related and Post-Menopause Migraine

doi: 10.3390/jcm10091854

Figure Lengend Snippet: Representative 2DE urinary maps. The differentially expressed protein spots among ( A ) control group, ( B ) menstrually related migraine group, and ( C ) post-menopause migraine group are evidenced by ellipses; protein entry names refer to those reported in . Two-dimensional separation was performed by 17 cm IPG strip, pH range 3–10 (first-dimension), and 8–16% polyacrylamide gradient gel (second-dimension). Molecular weight marker (PrecisionPlus All Blue, Bio-Rad) is expressed in kilodalton (kDa).

Article Snippet: Large size 8–16% polyacrylamide gradient gels (29:1 acrylamide/bis solution, 1.5 M Tris, pH 8.8, 10% SDS, 1% TEMED, 10% ammonium persulfate, from Bio-Rad Laboratories) and TGS 1X running buffer (Bio-Rad Laboratories) were employed for the electrophoretic run.

Techniques: Control, Stripping Membranes, Molecular Weight, Marker

Journal: Stem Cells International

Article Title: Preconditioning of Rat Bone Marrow-Derived Mesenchymal Stromal Cells with Toll-Like Receptor Agonists

doi: 10.1155/2019/7692973

Figure Lengend Snippet: Analysis of the effect of LPS preconditioning on the proregenerative ability of rBM-MSCs. (a–c) Photomontages showing the regenerated portions of the sciatic nerve that were inside the polyethylene tubes 4 weeks after transection. Axons were stained with TuJ1 antibodies (red). In the Vehicle group, only the Matrigel® solution was injected into the tube, while in the MSC and LPS-MSC groups, 5 × 10 5 rat bone marrow-derived mesenchymal stromal cells (rBM-MSCs) or lipopolysaccharide- (LPS-) preconditioned rBM-MSCs, respectively, were suspended in the Matrigel® solution and then injected into the tube immediately after sciatic nerve transection and tubulization. Scale bar: 200 μ m. (d–g) Quantitative analysis of nerve thickness, cell density, and number of TuJ1-positive axons (in the proximal and distal nerve segments) in each condition ( n = 5 rats per group; rBM-MSCs from only 1 animal were used in this experiment to reduce donor-related variability). Data are shown as individual values, median, and interquartile range. ∗ p < 0.05; Kruskal-Wallis test with Dunn's multiple comparisons test.

Article Snippet: 5 × 10 5 rBM-MSCs (or preconditioned rBM-MSCs), suspended in 15 μ L of a Matrigel® Growth Factor Reduced Basement Membrane Matrix (Corning) solution (1 : 3 in DMEM/F12 medium), were injected into the tube.

Techniques: Staining, Injection, Derivative Assay